Acetomicrobium hydrogeniformans

The Acetomicrobium hydrogeniformans is a part of the phylum Synergistetes which belongs to the class Synergistia and it is of the order Synergistales. It is a member of the family of Synergistaceae. Its genus is Acetomicrobium and it is of the Acetomicrobium hydrogeniformans species.

Acetomicrobium hydrogeniformans (Maune and Tanner 2012) Ben Hania et al. 2016

Scientific Classification
Full Scientific Name	Acetomicrobium hydrogeniformans (Maune and Tanner 2012) Ben Hania et al. 2016
Phylum	Synergistetes
Class	Synergistia
Order	Synergistales
Family	Synergistaceae
Genus	Acetomicrobium
Species	Acetomicrobium hydrogeniformans
Temperature Range	thermophilic
Country	USA
Continent	North America
साँचा:navbar

Culture and Growth conditions

Culture Media

A microbial culture medium is a mixture of substances that promotes and supports the growth and differentiation of microorganisms. Culture media contain nutrients, energy sources, growth-promoting factors, minerals, metals, buffer salts, and gelling agents (for solid media).

The culture media used for this sample is/are –

• ANAEROBACULUM MEDIUM (DSMZ Medium 104a)

The corresponding compositions of the culture media is/are –

• Name: ANAEROBACULUM MEDIUM (DSMZ Medium 104a)
  • Composition: Yeast extract 10.0 g/l, Trypticase peptone 5.0 g/l, Meat peptone 5.0 g/l, Na2S2O3 x 5 H2O 2.5 g/l, D-Glucose 1.0 g/l, L-Cysteine HCl x H2O 0.5 g/l, NaHCO3 0.4 g/l, NaCl 0.08 g/l, KH2PO4 0.04 g/l, K2HPO4 0.04 g/l, MgSO4 x 7 H2O 0.02 g/l, CaCl2 x 2 H2O 0.01 g/l, Sodium resazurin 0.0005 g/l, Distilled water

Metabolite Utilization

Bacteria produce several intermediates or metabolites during its metabolism. The different metabolites in Acetomicrobium hydrogeniformans and their utilization are -

Metabolite	Utilization
asparagine	carbon source
fructose	carbon source
glucose	carbon source
glutamate	carbon source
histidine	carbon source
leucine	carbon source
malonate	carbon source
maltose	carbon source
mannose	carbon source
phenylalanine	carbon source
pyruvate	carbon source
serine	carbon source
threonine	carbon source
glycerol	assimilation
D-fructose	assimilation
D-glucose	assimilation
L-tartrate	assimilation
maltose	assimilation
mannose	assimilation
pyruvate	assimilation

Temperature

Generally, an increase in temperature will increase enzyme activity. But if temperatures get too high, enzyme activity will diminish and the protein (the enzyme) will denature.

On the other hand, lowering temperature will decrease enzyme activity. At freezing temperatures enzyme activity can stop. Repeated cycles of freezing and thawing can denature proteins. In addition, freezing causes water to expand and also forms ice crystals, hence cells begin to rupture.

Every bacterial species has specific growth temperature requirements which is largely determined by the temperature requirements of its enzymes.

The temperature at which the samples of Acetomicrobium hydrogeniformans (Maune and Tanner 2012) Ben Hania et al. 2016 have been cultured is/are-

Temperature	Kind of temperature
55	growth
40 - 65	growth
55	optimum

Hence, from the above data, it is understood that the bacteria is thermophilic .Thermophiles are “heat-loving” organisms having optimum growth between 50-60°C. Many thermophiles cannot grow below 45°C..

pH

the pH range of Acetomicrobium hydrogeniformans (Maune and Tanner 2012) Ben Hania et al. 2016

pH	Kind of pH
06 - 9	growth
7	optimum

Enzymes

• catalase

• cytochrome oxidase

Isolation, sampling and environmental information

Isolation

Isolation refers to the separation of a strain from a natural, mixed population of living microbes, in order to identify the microbe of interest. This sample type is isolated from oil water separation unit effluent, Oil production water.

Location

The samples of Acetomicrobium hydrogeniformans (Maune and Tanner 2012) Ben Hania et al. 2016 have been obtained from Alaska, North Slope Borough, USA, North America.